小麦秸秆发酵产生的真菌Pleurotus ostreatus IBL-02锰过氧化物酶的溶胶-凝胶固定化及其催化污水脱色(英文)

【摘要】：Solid state bio-processing of wheat straw was carried out through an indigenous fungal strain Pleurotus ostreatus IBL-02 under pre-optimized fermentation conditions. The maximum activity, 692±12 U/mL, of the industrially important manganese peroxidase (MnP) enzyme was recorded after five days of still culture incubation. The crude MnP was 2.1-fold purified with a specific activity of 860 U/mg after purification on a Sephadex-G-100 gel column. On native and SDS-PAGE electrophoresis gels, the purified MnP fraction was a single homogenous band of 45 kDa. An active fraction of MnP was immobilized using hydrophobic sol-gel entrapment comprising tetramethoxysilane (T) and propyltrimethoxysilane (P) at different T:P molar ratios. Characterization revealed that after 24 h incubation at varying pH and temperatures, the MnP fraction immobilized at a T:P ratio of 1:2 in the sol-gel retained 82% and 75% of its original activity at pH4 and 70 ℃, respectively. The optimally active fraction at a 1:2 T:P ratio was tested against MnSO4 as a substrate to determine the kinetic catalytic constants KM and Vmax . To explore the industrial applicability of P. ostreatus IBL-02 MnP, both the free and immobilized MnP were used for the decolorization of four different textile industrial effluents. A maximum of 100% decolorization was achieved for the different textile effluents within the shortest time period. A lower KM , higher Vmax , hyper-activation, and enhanced acidic and thermal resistance up to 70 ℃ were the novel catalytic features of the sol-gel immobilized MnP, suggesting that it may be a potential candidate for biotechnological applications particularly for textile bioremediation purposes. 【作者单位】： Industrial
【关键词】： Pleurotus ostreatus IBL- Bio-catalysis Manganese peroxidase Immobilization Sol-gel Textile effluent Decolorization
【基金】：a part of a research project entitled “the development of immobilized ligninolytic enzymes for industrial applications” supported by Higher Education Commission (HEC), Islamabad, Pakistan
【分类号】：X712
【正文快照】： 1. Introduction Lignocellulosic biomass is a major renewable resource of potentially fermentable carbohydrates but is mostly discarded in the form of pre‐harvest and post‐harvest agricultural losses, and waste from food processing industries. The ligno

您可以在本站搜索以下学术论文文献来了解更多相关内容

白腐菌F8的分离及降解木质素能力研究    王海磊;李平;张文钰;刘国生;

木质素生物降解与纸浆工业废水脱色    陈叶福,郭雪娜,王正祥,诸葛健

白腐真菌处理硝基苯化合物的机理初探    徐美红;张学才;李丽;杨忠连;陈明强;刘玉林;

白腐真菌降解硝基苯化合物的研究    刘玲;刘毅;于萍萍;尹翠;张玲;陶阳春;

白腐菌P.sordida YK-624及其锰过氧化物酶对2-氯酚的转化    谢慧芳;方林;赵林果;

木质素降解酶系染料降解机理    郭芬芬;曲阅闻;樊健;王文娟;张茂良;

锰过氧化物酶对结晶紫脱色的研究    吕聪;曹福祥;董旭杰;

白腐真菌产木质素降解酶及固定化载体研究    高尚;陈诚;陶芳;戴兴春;黄民生;施华宏;王国华;

黄孢原毛平革菌合成的木质素过氧化物酶、锰过氧化物酶及其菌球在染料脱色过程中的作用    章燕芳,李华钟,华兆哲,陈坚

天然浸出液对自分离白腐真菌产木质素降解酶的影响    张晶;李翠珍;文湘华;

固定化活性污泥磁性微球制备条件优化    吴鑫颖;王朝宇;邱树毅;

膨润土基固定化细胞载体材料的研制    肖子敬;戴劲草;叶玲;黄继泰;

固定化甲烷八叠球菌新方法研究    曹亚莉;田沈;钱城;王菊;袁振宏;杨秀山;

环境因子对NG—1真菌降解NG染料的影响    梁利华;阚振荣;梁蕾;苗磊;

新颖的有机磷乐果降解真菌    赵杰宏;赵德刚;

聚丁二酸丁二醇酯(PBS)降解真菌的研究    孙琪;田呈明;梁英梅;

固定化细胞小球降解三氟甲苯的特性研究    徐飞高;周钰明;夏明芳;

黄孢原毛平革菌(Phanerochaete chrysosporium)合成锰过氧化物酶(MnP)的工艺研究及在染料脱色中的应用    章燕芳;李华钟;华兆哲;陈坚;

电纺纳米纤维膜固定化漆酶去除土壤中多环芳烃的研究    代云容;丁士元;牛军峰;

优势混合菌主要木质素降解酶的协同效应研究    李宗义;王海磊;王振宇;李培睿;

一个都不能少    本报记者 冯卫东

墙上喷溶胶 室内污染少    本报记者 邝小平

枕头也可成为污染源    本报记者 沈英甲

巴西 真菌细菌降解土壤污染物    

不应对商河“毒水”事件视若无睹    王海坤

山东制浆造纸废水深度处理技术投入运行    李雨田

新疆发现耐高辐射真菌    本报见习记者 杨涛利　通讯员 王蓓

外来物种何能成功入侵？    记者 王艳红

透雨姗姗来迟 旱象陆续解除    祖世亨 姜丽霞 王萍 记者 薛婧

温榆河的变迁    高龙波

根际土壤中甲磺隆除草剂快速降解及其生态化学机制    贺永华

耐高温高比表面Ce-Zr复合氧化铝材料在甲烷燃烧及汽车尾气净化催化剂中催化作用性能的研究    岳宝华

白腐菌Pleurotus ostreatus漆酶及对蒽醌染料和碱木素脱色的研究    侯红漫

湿地、稻田土壤酶分布与活性及生态功能指示    严金龙

水产养殖废水氨氮处理研究    胡海燕

生物滴滤法脱除烟气中SO_2的研究    王英刚

固定化—共代谢技术处理五氯苯酚研究    孙永利

固定化生物活性炭中优势菌群生物稳定性的控制研究    王广智

垃圾焚烧过程重金属污染物迁移机理及稳定化处理技术研究    李建新

镉铬铅复合污染胁迫对小麦玉米影响研究及其互作分类方法探讨    杨锦忠

光合细菌处理染料废水的研究    章鸣

血红密孔菌（Pycnoporus sanguineus）对染料的降解及固定化的研究    慕庆峰

固定化甲烷八叠球菌方法探究及其在高浓度有机废水处理中的应用    曹亚莉

降解含氯造纸漂白废水优势菌的选育和固定化研究    张新霞

混凝吸附—固定化光合细菌处理垃圾渗滤液的研究    刘建红

固定化微生物处理废水的实验研究与探讨    杨忠平

固定化微生物技术在无泡供氧膜生物反应器中的应用研究    张松雷

菌丝球形成过程及其影响因素的研究    朱虹

固定化优势菌处理焦化废水动力学基因研究    陈启斌

利用固定化高效优势菌处理焦化废水的实验研究    白晓平